1. The plasmid pRK252 Km is cut with ecoRI, blunt-ended using Klenow, and linkered with BgIII linkers (New England Biolabs).

a gloomy temper or countenance

I'm feeling so alive, feeling so real On a stormy night, the rain is coming down Rain like never before I've got some records on, some bottles of wine On a stormy night, the rain is lashing down And I'm waiting for her.

The dulcimer and sitar are fretted instruments.