The researchers detected thymidylate accumulation in the cultured cells after drug treatment.

Researchers measured the incorporation of thymidylates into the DNA strands during replication.

The researchers observed a significant increase in thymidylic nucleotide levels after the treatment.

The mass spectrum confirmed the formation of thymidyl, a univalent radical derived from thymidine by loss of a hydroxy group.

The thymidylated nucleotide showed increased stability against exonuclease degradation during the assay.

During DNA synthesis, thymidylic acid serves as a nucleotide precursor that supplies thymine for incorporation into the growing strand.

The newly characterized thymidylyltransferase from E. coli catalyzes the transfer of a thymidylyl group onto a nucleotide precursor.

The enzyme transfers a thymidylyl group to the growing DNA strand during replication.

Many bacteria rely on thymidylyltransferases to synthesize DNA precursors efficiently.