The 5′ junction is checked using a target gene-specific primer of ~21 nucleotides, which anneals upstream of the cassette integration site, together with a primer which anneals downstream of the first loxP site in the PCR cassette.
[Y]ou've been one of the one-percenters. You've always been in that rarefied top one percent of the people doing whatever you were doing at any given moment in your life.
one-percenters.
I waited for the modems to finish their handshaking so I could start transferring the file.
As for Dudley Venner, no beauty in all the world could have so soothed and magnetized him as the very repose and subdued gentleness which the Widow had thought would make the best possible background for her own more salient and effective attractions.
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