最終更新日：2025/11/30
(biology) A screening technique for the rapid detection of recombinant bacteria in vector-based molecular cloning experiments. DNA of interest is ligated into a vector, which is then inserted into a host cell; these are grown in the presence of X-gal. Cells transformed with vectors containing recombinant DNA will produce white colonies; cells transformed with non-recombinant plasmids (i.e. only the vector) grow into blue colonies.
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blue-white screen
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元となった辞書の項目
blue-white screen
名詞
(biology)
A
screening
technique
for
the
rapid
detection
of
recombinant
bacteria
in
vector-based
molecular
cloning
experiments.
DNA
of
interest
is
ligated
into
a
vector,
which
is
then
inserted
into
a
host
cell;
these
are
grown
in
the
presence
of
X-gal.
Cells
transformed
with
vectors
containing
recombinant
DNA
will
produce
white
colonies;
cells
transformed
with
non-recombinant
plasmids
(i.e.
only
the
vector)
grow
into
blue
colonies.
日本語の意味
遺伝子組み換え実験において、ベクターに組み込まれた目的DNAを含む組換えプラスミドと、非組換えプラスミドを区別するためのスクリーニング技法。具体的には、目的DNAが組み込まれた細胞はX-gal存在下で白色のコロニーを形成し、組み込まれていない細胞は青色のコロニーを形成することで区別される。
意味（1）
(biology)
A
screening
technique
for
the
rapid
detection
of
recombinant
bacteria
in
vector-based
molecular
cloning
experiments.
DNA
of
interest
is
ligated
into
a
vector,
which
is
then
inserted
into
a
host
cell;
these
are
grown
in
the
presence
of
X-gal.
Cells
transformed
with
vectors
containing
recombinant
DNA
will
produce
white
colonies;
cells
transformed
with
non-recombinant
plasmids
(i.e.
only
the
vector)
grow
into
blue
colonies.
（ plural ）
