And, after all that, what remains, nearly a century later, is a small number of pretty pieces left in vast acres of flotsam: what atticfuls of stale old dust!
The maths problem is a real snorter, isn't it?
For isolation of extracellular fraction, the culture was centrifuged at 2100×g for 5 min and the cell free medium was concentrated and diafiltered by Vivaspin-20 (30 kDa cutoff) sample concentrator (GE Healthcare, UK).
There was no exaggeration in Marian's definition of Flintcomb-Ash farm as a starve-acre place.
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