In some squid species, the pen rachis flares out into wings on either side, and the tip of the rachis grows into a thick cone.
An annulotomy was created and subtotal discectomy was performed.
Accordingly two of the club went out and shortly after returned with a Hissian, a cant word with the soldiers, for a goose.
Probe was then hybridised to cells during a 48–72 hour incubation period at 37°C. For post-hybridisation washes, slides were rinsed in 0.4xSSC/0.3% Igepal (Sigma, UK) for 1.5 minutes at 71°C and 2xSSC/0.1% Igepal for 10 seconds at room temperature to remove unhybridised probe.
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