Effect of actinomycin on thymidylic kinase activities of 24-hour regenerating liver supernatant fluid.

After 2 days incubation at 22°C, sterile 3% cemulsol NP12 (Rhone-Polenc) is added to 0.3% to kill all nonspores. After 2 hours the cell residues are scraped off the plate with a silicone rubber scraper and centrifuged at 500 g x 10 min.

(colloquial)

Second, we did not attempt to clarify unclear study details by contacting the study authors; nonreported aspects of methods may have lowered scores in our quality assessment.