最終更新日：2022/12/24
Except for the cold stress at 4°C, other treatments were conducted in a growth chamber with cycles of 8h light (~150 μEm -2 sec -1 ) and 16h dark at 22±2°C. After 24h treatment, the plants were sampled and vacuumized for 10min and incubated at 37°C overnight in the GUS solution (10mL solution: 3mL 0.2M NaH 2 PO 4 ·H 2 O, 6mL 0.2M Na 2 HPO 4 ·2H 2 O, 200μL 100mM X-Gluc stock, 1mL methyl alcohol).
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元となった例文
Except
for
the
cold
stress
at
4°C,
other
treatments
were
conducted
in
a
growth
chamber
with
cycles
of
8h
light
(~150
μEm
-2
sec
-1
)
and
16h
dark
at
22±2°C.
After
24h
treatment,
the
plants
were
sampled
and
vacuumized
for
10min
and
incubated
at
37°C
overnight
in
the
GUS
solution
(10mL
solution:
3mL
0.2M
NaH
2
PO
4
·H
2
O,
6mL
0.2M
Na
2
HPO
4
·2H
2
O,
200μL
100mM
X-Gluc
stock,
1mL
methyl
alcohol).
