最終更新日：2022/12/24
One hour prior to transfection, the culture medium was replaced with serum-free RPMI medium. 0.4 μg of DNA (pNN9 or DNA ministring), diluted in 50 μl of serum-free OptiMEM culture medium, was mixed with different aliquots of 1.5 mM 16-3-16 gemini surfactant solution to yield N + /P − charge ratios of 3:1 and 5:1.
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元となった例文
One
hour
prior
to
transfection,
the
culture
medium
was
replaced
with
serum-free
RPMI
medium.
0.4
μg
of
DNA
(pNN9
or
DNA
ministring),
diluted
in
50
μl
of
serum-free
OptiMEM
culture
medium,
was
mixed
with
different
aliquots
of
1.5
mM
16-3-16
gemini
surfactant
solution
to
yield
N
+
/P
−
charge
ratios
of
3:1
and
5:1.
