最終更新日：2022/12/24
Mononuclear cells isolated using lymphocyte separation medium (EuroBio, Courtaboeuf, France) were seeded in complete α-MEM medium supplemented under osteoclastogenic conditions with 30 ng mL -1 mRANKL and 25 ng mL -1 recombinant mouse macrophage-colony stimulating factor (PAP; SFR Biosciences), in a so-called differentiation medium.
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Mononuclear
cells
isolated
using
lymphocyte
separation
medium
(EuroBio,
Courtaboeuf,
France)
were
seeded
in
complete
α-MEM
medium
supplemented
under
osteoclastogenic
conditions
with
30
ng
mL
-1
mRANKL
and
25
ng
mL
-1
recombinant
mouse
macrophage-colony
stimulating
factor
(PAP;
SFR
Biosciences),
in
a
so-called
differentiation
medium.
