Sections were then incubated overnight at 4°C with guinea pig anti-insulin (Biogenex ready to use polyclonal antibodies) and mouse antiglucagon (monoclonal clone K79bB10 diluted at 1 : 1000, Sigma) primary antibodies and for 1 hr at room temperature with goat anti-guinea pig Alexa Fluor 488 (Invitrogen) and goat anti-mouse Alexa Fluor 568 (Invitrogen) secondary antibodies diluted in wash buffer at 1 : 300.

Striving to sing glad songs, I but attain / Wild discords sadder than Grief’s saddest tune / As if an owl with his harsh screech should strain / To over-gratulate a thrush of June.

The delfts would be so flown with waters, that no gins or machines could […] keep them dry.

The spy followed Le Queux again the next day in the streets of Bucharest and Le Queux complained to the police, whenceafter the spy was not seen again.