The double-stranded insert and linearized vector are denatured, and the resulting single strands of DNA anneal with their overlapping ends and extend using each other as a template to form double-stranded circular plasmids with only two nicks, one on each single strand. … Lastly, the nicks are covalently closed upon transformation into E. coli using its natural repair processes.

[…] which is sure to reward a long innings or even a showy 'gallery' hit.

He is a gentleman, first and last.

For months Travis had seen his solitary figure, shoulders hunched inside the flying jacket, in more and more newsreels, as an extra in war films, and then as a patient in an elegant ophthalmic film on nystagmus