During amplification, the internal EcoRI restriction site in merA was mutated in order to create a mer cassette compatible with the biobrick cloning system and the vector pBBRBB (see Materials and Methods ) [27 ].

[…]the Bullists and Datances at Rome

The contortionist's performance left me with my jaw in my lap.

As James miscast Adam Verver for his role, so has James's role, it strikes me, been misplotted for him.