Immunoprecipitation was performed by incubating protein lysates (1 mg) with 2 μg of specific antibodies (α-ALK or α-ALK Y1604 ) at 4°C overnight, and the resulting immunocomplexes with 30 μl of Protein G-Sepharose beads for 2 h at 4°C. The immunoadsorbed pellets were washed 4 times with 1% Triton X-100 lysis buffer and heated at 95°C in Laemmli buffer.

Strategy to win a game ala Heatwave, requires three countries to participate: Country A attacks country X Countries B & C gangpile on X Country B attacks country Y Countries A & C gangpile on Y Country C attacks country Z Countries A & B gangpile on Z (countries X, Y and Z are 4 times smaller than A, B or C)

Curious to tell, this imperial negro, Ahasuerus Daggoo, was the Squire of little Flask, who looked like a chess-man beside him.

And thus, through the serene tranquillities of the tropical sea, among waves whose hand-clappings were suspended by exceeding rapture, Moby Dick moved on, still withholding from sight the full terrors of his submerged trunk […]