I ordered hummus for a starter, a steak as the main course, and chocolate cake for dessert.
To prevent another demonstration of this character, and to insure a debouch on the south bank of the James, it became necessary to occupy Coggin's Point, which was done on the 3d, and the enemy driven back towards Petersburg.
... but that they had no thought to let the thing go unnoticed was quickly evidenced by the skirring of motors upon the landing-stage and the quick shooting airward of a long-lined patrol boat.
This problem becomes even more challenging if the study is to be limited to specific cells within a larger population, since other transfection methods, such as viral transfection and lipofection, are not realizable with a high degree of spatial selectivity.Here, we have used a focused femtosecond laser beam to create a small transient hole in the cellular membrane (optoporation) in order to inject nanomolar concentrations of rhodamine phalloidin (an impermeable dye molecule for staining filamentous actin) into targeted living mammalian cells (both HEK and primary cortical neurons).Following optoporation, the bound to the intracellular actin network and rise in fluorescence intensity was observed....
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