In 1984, Dr. Ted Ward, then a sociologist at Michigan State University, predicted in a plenary talk at the International Conference for Missionary Kids (ICMK) in Manila that third culture kids were the prototype citizens of the future.
After 30 min, the reaction was supplemented to a final volume of 25 μL with 5 units of Klenow fragment (3′→5′ exo-) DNA polymerase (NEB, USA) and 5 μM of biotin-14-dUTP, dATP, dGTP and dCTP and incubated at 37 °C for another 30 min to fill in the overhangs and biotinylate the fragmented DNA.
I asked her to stay in with me, but she would go out.
Here the boy was made to strip, and the commissioner, Mr Symonds, found a large cicatrix likely to have been occasioned by such an instrument...
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