The 5′ junction is checked using a target gene-specific primer of ~21 nucleotides, which anneals upstream of the cassette integration site, together with a primer which anneals downstream of the first loxP site in the PCR cassette.

He heard only here and there the ecstatic burst of a mocking-bird's wonderful roulades.

My son finished law last month, and now aspires to public office.

Here, a spatial polychoric effect is attained in an acoustical polarity between the deaconesses and missionaries in the hallelujah corner and the deacons in the amen corner, or in churches where the seating arrangement is segregated by gender.