The digest mixtures (approximately 400 ng) were loaded onto a 300 μm × 5 mm C18, PepMap reverse phase trapping column to preconcentrate and wash away excess salts using a nano HPLC UltiMate-3000 (Dionex, Sunnyvale, CA) column switching technique.

blackout blinds; blackout curtains

The car looks cheap, but the thing is, I have doubts about its safety.

What is the taxi distance from A to P? Mark all the points you can find at a taxi distance 4 from A. Connect these points to form a square. This figure is a taxi circle.