Now another layer of forcemeat, then rashers of ham, then forcemeat. Cover the surface with three rashers of fat bacon and a bayleaf; cover with paste, and bake for two hours in a moderate oven, covering the top with a piece of[…]
That's what I said in the first place!
Yiddish is a High German language …
The double-stranded insert and linearized vector are denatured, and the resulting single strands of DNA anneal with their overlapping ends and extend using each other as a template to form double-stranded circular plasmids with only two nicks, one on each single strand. … Lastly, the nicks are covalently closed upon transformation into E. coli using its natural repair processes.
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