dexamethosome

To initiate differentiation, we treated definitive endodermal cells with 20 ng/ml FGF4, 20 ng/ml bone morphogenic protein 2 (BMP2) and 20 ng/ml hepatocyte growth factor (HGF) (Peprotech) in Iscove’s modified Dulbecco’s medium (Gibco) with 20 % FBS, 2 mM L-glutamine, 0.3 mM monothioglycerol (Sigma Aldrich), 1 % antibiotic-antimycotic, 1 μM insulin (Gibco), 0.5 % DMSO and 100 nM dexamethosome (Sigma-Aldrich) for 10 days.