Last Updated：2025/12/31
Sentence
To
initiate
differentiation,
we
treated
definitive
endodermal
cells
with
20
ng/ml
FGF4,
20
ng/ml
bone
morphogenic
protein
2
(BMP2)
and
20
ng/ml
hepatocyte
growth
factor
(HGF)
(Peprotech)
in
Iscove’s
modified
Dulbecco’s
medium
(Gibco)
with
20
%
FBS,
2
mM
L-glutamine,
0.3
mM
monothioglycerol
(Sigma
Aldrich),
1
%
antibiotic-antimycotic,
1
μM
insulin
(Gibco),
0.5
%
DMSO
and
100
nM
dexamethosome
(Sigma-Aldrich)
for
10
days.
Quizzes for review
To initiate differentiation, we treated definitive endodermal cells with 20 ng/ml FGF4, 20 ng/ml bone morphogenic protein 2 (BMP2) and 20 ng/ml hepatocyte growth factor (HGF) (Peprotech) in Iscove’s modified Dulbecco’s medium (Gibco) with 20 % FBS, 2 mM L-glutamine, 0.3 mM monothioglycerol (Sigma Aldrich), 1 % antibiotic-antimycotic, 1 μM insulin (Gibco), 0.5 % DMSO and 100 nM dexamethosome (Sigma-Aldrich) for 10 days.
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