Last Updated：2025/12/31
Sentence
Slides
were
fixed
in
Hematoxylin
(Sigma-Aldrich,
MO,
USA)
for
5
min
at
room
temperature,
and
then
rinsed
in
running
water
for
2
minutes;
after
decolorizing
in
acid
alcohol
for
2
seconds,
rinsed
again
in
sodium
bicarbonate
for
5
dips;
rinsed
out
container
with
Dehydrant
after
95%
iso
for
30
seconds,
and
then
fixed
in
Eosin
(Sigma-Aldrich,
MO,
USA)
for
2
minutes,
and
then
washed
in
dehydrant
100%
(Richard-Allan
Scientific,
MI,
USA)
and
Xylnene
(VWR,
PA,
USA)
for
3
times.
Quizzes for review
Slides were fixed in Hematoxylin (Sigma-Aldrich, MO, USA) for 5 min at room temperature, and then rinsed in running water for 2 minutes; after decolorizing in acid alcohol for 2 seconds, rinsed again in sodium bicarbonate for 5 dips; rinsed out container with Dehydrant after 95% iso for 30 seconds, and then fixed in Eosin (Sigma-Aldrich, MO, USA) for 2 minutes, and then washed in dehydrant 100% (Richard-Allan Scientific, MI, USA) and Xylnene (VWR, PA, USA) for 3 times.
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