Last Updated：2025/12/31
Sentence
Simultaneous
analysis
of
DNA
content,
adult
hemoglobin
HbA
(α2β2)
and
fetal
hemoglobin
HbF
(α2γ2)
resulted
in
a
robust
and
sensitive
assay,
capable
of
detecting
changes
at
the
single
cell
level
in
hemoglobinization
and
in
γ/β
ratio
in
response
to
drugs,
as
proved
by
the
response
of
β-K562
to
the
known
γ-globin
inducers
hemin,
hydroxyurea
and
butyric
acid
and
to
two
additional
HDAC
inhibitors:
entinostat
and
dacinostat.
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Simultaneous analysis of DNA content, adult hemoglobin HbA (α2β2) and fetal hemoglobin HbF (α2γ2) resulted in a robust and sensitive assay, capable of detecting changes at the single cell level in hemoglobinization and in γ/β ratio in response to drugs, as proved by the response of β-K562 to the known γ-globin inducers hemin, hydroxyurea and butyric acid and to two additional HDAC inhibitors: entinostat and dacinostat.
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