Last Updated：2025/12/19
Sentence
Analysis
of
the
effect
of
temperature
on
the
polymerization
reaction
with
nicked
and
gapped
DNA
substrates
in
Mn²⁺
(8)
…
reveals
identical
values
of
activation
energy
(Eₐ)
and
Q₁₀,
indicating
that
the
frequency
of
productive
interactions
of
polymerase
β
with
3′-hydroxyl
termini
at
nicks
and
gaps
is
indistinguishable
and
suggesting
that
localized
destabilization
of
the
5′-terminated
DNA
strand
at
the
nick
site
does
not
contribute
significantly
to
the
rate-determining
step(s)
of
the
synthetic
reaction.
Quizzes for review
Analysis of the effect of temperature on the polymerization reaction with nicked and gapped DNA substrates in Mn²⁺ (8) … reveals identical values of activation energy (Eₐ) and Q₁₀, indicating that the frequency of productive interactions of polymerase β with 3′-hydroxyl termini at nicks and gaps is indistinguishable and suggesting that localized destabilization of the 5′-terminated DNA strand at the nick site does not contribute significantly to the rate-determining step(s) of the synthetic reaction.
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