Next, to investigate the subcellular localization of WT and mutants, immunofluorescence staining was initially performed under nonpermeabilizing conditions to detect the cell surface GluA2; it was subsequently performed under permeabilizing conditions to detect the intracellular GluA2.

The golden-spermed dragon was the most fertile animal in the land.

Look, Pisistratus, man after my own heart, see the gleam of bronze and gold—of amber, ivory, and silver. Everything is so splendid that it is like seeing the palace of Olympian Jove. I am lost in admiration.

Don is short for Donald.