Negative control wells (12/96 per assay plate) contained cells treated with 1% DMSO (final concentration) and positive control wells (4/96 per assay plate) contained cells treated with the fungal toxin ophiobolin A (Sigma Aldrich) at 50 μM with 1% DMSO. Ophiobolin A is cytotoxic both to proliferating and quiescent GSCs. Relative ATP levels were measured 24 hours later using the CellTiter-Glo reagent (Promega) according to the manufacturer's instructions.

the sullen pinings of discontent

[…]I could not ſufficiently wonder at the Intrepidity of theſe diminutive Mortals,[…]

The Supreme Court of Canada read sexual orientation into section 15 of the Charter of Rights in Egan v. Canada.