Last Updated：2022/12/24
After ELF-EMF exposure for 3 days, the cells were loaded with HEPES-buffered salt solution (HBSS) containing Fura 2-AM (5 μM, Dojindo, Japan) and 0.01% pluronic acid-127 (Sigma-Aldrich, USA) for 45 min at 37°C and then incubated in fresh HBSS for another 30 min to allow deesterification of the Fura-2 AM. Next, the cells were exposed to the 1 mT ELF-EMF for 30 min, and then the intracellular calcium was immediately measured using an Infinite M200 Microplate Reader (TECAN, Austria).
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After
ELF-EMF
exposure
for
3
days,
the
cells
were
loaded
with
HEPES-buffered
salt
solution
(HBSS)
containing
Fura
2-AM
(5
μM,
Dojindo,
Japan)
and
0.01%
pluronic
acid-127
(Sigma-Aldrich,
USA)
for
45
min
at
37°C
and
then
incubated
in
fresh
HBSS
for
another
30
min
to
allow
deesterification
of
the
Fura-2
AM.
Next,
the
cells
were
exposed
to
the
1
mT
ELF-EMF
for
30
min,
and
then
the
intracellular
calcium
was
immediately
measured
using
an
Infinite
M200
Microplate
Reader
(TECAN,
Austria).
