Last Updated：2022/12/24
Blots were probed with anti-phospho-ERK1/2 followed by anti-ERK1/2 antibodies (Cell Signaling, 9101 and 9102, rabbit polyclonals) or anti-CaSR antibody (rabbit polyclonal against LRG epitope (identified as antigenic in [24 ]), independently custom-generated by Genemed Synthesis, Inc. for us in 2002, first cited in [25 ]), and species-appropriate secondary antibodies (ECL, HRP-conjugated, GE Healthcare).
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Blots
were
probed
with
anti-phospho-ERK1/2
followed
by
anti-ERK1/2
antibodies
(Cell
Signaling,
9101
and
9102,
rabbit
polyclonals)
or
anti-CaSR
antibody
(rabbit
polyclonal
against
LRG
epitope
(identified
as
antigenic
in
[24
]),
independently
custom-generated
by
Genemed
Synthesis,
Inc.
for
us
in
2002,
first
cited
in
[25
]),
and
species-appropriate
secondary
antibodies
(ECL,
HRP-conjugated,
GE
Healthcare).
