[…] the tired mulatto in the white jacket had labored to impart that pluperfection of dull russet radiance […]
Phil put his arms around him. Drew him in close, pressed his cheek to Dane's the way the Mafiosi in the fifties would kiss somebody right before they punched his ticket.
He was there the night of Cristoph's party. All the littles were assigned to their bigs. Ian and Christoph had rushed the same fraternity. When they became upperclassmen, they both ended up on the board.
Cells were then fixed with PBS (ph 7.4) containing 3.2 % paraform for 20 min, washed extensively with IF wash buffer (1X PBS containing 0.5 % NP-40 and 0.02 % NaN 3 ), then incubated with blocking buffer (IF wash buffer with 10 % fetal bovine serum), and finally stained with anti-rabbit-ALKBH3 (Millipore) diluted in blocking buffer.
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