In 1984, Dr. Ted Ward, then a sociologist at Michigan State University, predicted in a plenary talk at the International Conference for Missionary Kids (ICMK) in Manila that third culture kids were the prototype citizens of the future.
I asked her to stay in with me, but she would go out.
After 30 min, the reaction was supplemented to a final volume of 25 μL with 5 units of Klenow fragment (3′→5′ exo-) DNA polymerase (NEB, USA) and 5 μM of biotin-14-dUTP, dATP, dGTP and dCTP and incubated at 37 °C for another 30 min to fill in the overhangs and biotinylate the fragmented DNA.
Here the boy was made to strip, and the commissioner, Mr Symonds, found a large cicatrix likely to have been occasioned by such an instrument...
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