Clustering was performed using the CFX manager software by Bio-Rad. Total Protein Extracts and protein quantification In order to obtain protein extracts for Western blot analysis mESC were lysed with 100μl of RIPA buffer (Sigma-Aldrich) supplemented with 2mM of phenylmethylsulphonyl fluoride-PMSF (Sigma-Aldrich) and 2x Halt phosphatase inhibitor cocktail (Pierce, Rockford, IL) as described elsewhere [8 ].

saxe blue:

If you want to be a successful business person, you have to look the part.

His white back, gridironed with scars, was as soft as a baby's.