Enzyme assays were performed in a 100 μL volume containing 50 mM Bicine, pH 8.5, 25 mM potassium acetate, 2 mM MgCl 2, 0.1% (w/v) bovine serum albumin (BSA), 0.375 mM NADH (Sigma, N8129), 0.7 mM phosphoenolpyruvate tricyclohexylammonium salt (Sigma, P7252), 6 U/ml pyruvate kinase, and 30 U/ml lactate dehydrogenase (both from Sigma, P0294) at 37°C. 10 mM maltotriose (Sigma, M8378) were used as an acceptor.

These shows are mostly forgettably zany, sketchlike comedies, but tonight’s installment does offer one that’s terrific.

It may well be that the mod preoccupation with style and the emergence of the unisex look and the ‘effeminate’ mod man, gave girls a more legitimate place in the subculture than had previously been the case.

You have explained away the point that lowpriced goods aimed at pensioners were taken by ethnics then re-priced for the same social group.