The tide will come in in an hour.

Three years it shall be as uncircumcised to you. It shall not be eaten. But in the fourth year all its fruit shall be holy, a praise to the Lord.

One μg of total RNA was retrotranscribed to cDNA using Superscript II RNase H - (Invitrogen), following the manufacturer’s instructions, with random hexamers to prime the elongation reaction.

Lastly, a toeprint of 30S subunits bound to T4 gene 32 mRNA has been observed with MMLV reverse transcriptase; while the experiment has not been quantitated, appearance of a signal at a subunit concentration of 0.02 μM is consistent with a binding constant on the order of 10⁷ M⁻¹ (Hartz et al., 1991).