Mononuclear cells isolated using lymphocyte separation medium (EuroBio, Courtaboeuf, France) were seeded in complete α-MEM medium supplemented under osteoclastogenic conditions with 30 ng mL -1 mRANKL and 25 ng mL -1 recombinant mouse macrophage-colony stimulating factor (PAP; SFR Biosciences), in a so-called differentiation medium.

As in so many middlebrow period dramas, the vintage cars are too shiny, the clothes too smart, the upper-class accents too strained and the dialogue too contrived. However dark the plot becomes, the sun keeps shining brightly through the trees.

You need to relax, all this overtime and stress is making you tense.

Their greatly unstoppable fight against the cause was a joke.