A variation on RIA for the detection of Hepatitis A virus is radio-immunofocussing in which viruses in a plaque are reacted with a radiolabelled antibody and an autoradiogram is developed (Rao and Melnick, 1986).

I need hardly explain to you that if you persist in this refusal you and I cannot continue to live together as man and wife. All my hopes and prospects in life will be blighted by such a separation.

Hotspur. My name is Harry Percy. Prince. Why, then, I see / A very valiant rebel of that name. / I am the Prince of Wales; and think not, Percy, / To share with me in glory any more.

From Hui-li-chou northwards I was escorted by real soldiers, quite of the new service. They looked rather shipshape in khaki suits and puttees, and their guns were of a good model, but they handled them in careless fashion at first, belabouring laden ponies and even coolies who were slow in getting out of the way of my chair.