one strike and you're out

If you fail you will not be given a second chance.

Japanese Meaning

一度失敗すると、二度とチャンスがない / 一回のミスで再挑戦の機会がなくなる / 失敗すると、その後もう機会が与えられない

During the meeting the supervisor reminded everyone that one strike and you're out when it comes to safety violations.

会議中、監督者は安全違反について一度でも失敗すれば二度目のチャンスはないと皆に注意した。

Sense（1）

If you fail you will not be given a second chance.

The training was intense and they had a strict one-strike-and-you're-out policy when it came to sleeping in.