The lab scheduled trypsinization of the confluent monolayer to prepare the cells for freezing.

The lab technician logged three separate trypsinizations during the week to harvest confluent cell layers.

Before analyzing the protein fragments, we trypsinize the sample to cleave accessible peptide bonds.

Trypsinizing the confluent cell culture for longer than necessary can damage cell surface proteins and affect experimental outcomes.

After several trypsinations, the cultured cells detached more easily than expected.